Below protocols describe different ways to digest 1µL human blood plasma with products from ProteomEdge.
All protocols have been tested and validated for multiplexed absolute quantification of blood plasma proteins using Mass Spectrometry and products from ProteomEdge
Digestion RapiGest.pdf
Digestion SDC.pdf
Digestion TCEP.pdf
For for dissolving panel of protein fragments to be able to for example continue with digestion outside of the product plate, follow below protocol.