References

Read the articles describing the technique behind our products:

Kotol D, Hober A, Strandberg L, Svensson AS, Uhlén M, Edfors F. Targeted proteomics analysis of plasma proteins using recombinant protein standards for addition only workflows.

Biotechniques. 2021;71(3):473-483. doi:10.2144/btn-2021-0047

 

Zeiler M, Straube WL, Lundberg E, Uhlen M, Mann M. A Protein Epitope Signature Tag (PrEST) Library Allows SILAC-based Absolute Quantification and Multiplexed Determination of Protein Copy Numbers in Cell Lines.

Molecular & Cellular Proteomics. 2012;11(3):O111.009613. doi:10.1074/mcp.O111.009613

Data on the Human Protein Atlas:

Targeted mass-spectrometry based proteomics using our DiscoveryEdge175 panel was applied to a longitudinal pregnancy cohort.

The cohort comprised serial plasma samples collected from pregnant women in each trimester (first, second and third) to capture dynamic proteome changes over gestation. The measurements demonstrated high analytical reproducibility, with a median coefficient of variation of 7.5%, achieved without any batch normalization.

Read more and see all data on the Human Protein Atlas. 

Further reading

  1. Hober A., Henricsson M., Ruckh T., Davidsson P., Challis B., Miliotis T. (2025). Quantitative Assessment of a Novel Device Designed for Patient-centric Sampling of Dried Plasma Using Targeted Proteomics. Analytical Chemistry 2025, 97, 25 doi: 10.1021/acs.analchem.4c05455
  2. Johansson E., Iglesias MJ., Odeberg J. & Edfors F. (2025). Shaping the future of precision medicine: plasma proteomics to uncover insights in thrombosis. doi:10.1080/14789450.2025.2517146
  3. Villanueva Raisman, A., Kotol, D., Altay, O., Mardinoglu, A., Atak, D., Yurdaydin, C., … & Edfors, F. (2025). Advancing Chronic Liver Disease Diagnoses: Targeted Proteomics for the Non-Invasive Detection of Fibrosis. Livers, 5(1), 2. doi:10.3390/livers5010002
  4. Iglesias MJ, Sanchez-Rivera L, Ibrahim-Kosta M, et al. Elevated plasma complement factor H related 5 protein is associated with venous thromboembolism. Nat Commun. 2023;14(1):3280. doi:10.1038/s41467-023-38383-y
  5. Kotol D, Woessmann J, Hober A, et al. Absolute Quantification of Pan-Cancer Plasma Proteomes Reveals Unique Signature in Multiple Myeloma. Cancers (Basel). 2023;15(19):4764. doi:10.3390/cancers15194764
  6. Hober A, Rekanovic M, Forsström B, et al. Targeted proteomics using stable isotope labeled protein fragments enables precise and robust determination of total apolipoprotein(a) in human plasma. PLoS One. 2023;18(2):e0281772. doi:10.1371/journal.pone.0281772
  7. Woessmann J, Kotol D, Hober A, Uhlén M, Edfors F. Addressing the Protease Bias in Quantitative Proteomics. J Proteome Res. 2022;21(10):2526-2534. doi:10.1021/acs.jproteome.2c00491
  8. Malm M, Kuo CC, Barzadd MM, et al. Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins. Metab Eng. 2022;72:171-187. doi:10.1016/j.ymben.2022.03.009
  9. Karlsson MJ, Costa Svedman F, Tebani A, et al. Inflammation and Apolipoproteins Are Potential Biomarkers for Stratification of Cutaneous Melanoma Patients for Immunotherapy and Targeted Therapy. Cancer Res. 2021;81(9):2545-2555. doi:10.1158/0008-5472.CAN-20-2000
  10. Kotol D, Hunt H, Hober A, et al. Longitudinal Plasma Protein Profiling Using Targeted Proteomics and Recombinant Protein Standards. J Proteome Res. 2020;19(12):4815-4825. doi:10.1021/acs.jproteome.0c00194
  11. Hong MG, Dodig-Crnković T, Chen X, et al. Profiles of histidine-rich glycoprotein associate with age and risk of all-cause mortality. Life Sci Alliance. 2020;3(10):e202000817. doi:10.26508/lsa.202000817
  12. Edfors F, Forsström B, Vunk H, et al. Screening a Resource of Recombinant Protein Fragments for Targeted Proteomics. J Proteome Res. 2019;18(7):2706-2718. doi:10.1021/acs.jproteome.8b00924
  13. Andersson A, Remnestål J, Nellgård B, et al. Development of parallel reaction monitoring assays for cerebrospinal fluid proteins associated with Alzheimer’s disease. Clinica Chimica Acta. 2019;494:79-93. doi:10.1016/j.cca.2019.03.243
  14. Hober A, Edfors F, Ryaboshapkina M, et al. Absolute Quantification of Apolipoproteins Following Treatment with Omega-3 Carboxylic Acids and Fenofibrate Using a High Precision Stable Isotope-labeled Recombinant Protein Fragments Based SRM Assay. Molecular & Cellular Proteomics. 2019;18(12):2433-2446. doi:10.1074/mcp.RA119.001765
  15. Edfors F, Boström T, Forsström B, et al. Immunoproteomics Using Polyclonal Antibodies and Stable Isotope–labeled Affinity-purified Recombinant Proteins. Molecular & Cellular Proteomics. 2014;13(6):1611-1624. doi:10.1074/mcp.M113.034140
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